| Rt Hon Margaret Mary Beckett | 8 June 2001 | 5 May 2006 |
| Rt Hon David Wright Miliband | 5 May 2006 | 28 June 2007 |
| Rt Hon Hilary James Wedgwood Benn | 28 June 2007 | 11 May 2010 |
| Mary Elizabeth Truss | 15 July 2014 | 14 July 2016 |
| Andrea Jacqueline Leadsom | 14 July 2016 | 11 June 2017 |
| Rt Hon Michael Andrew Gove | 11 June 2017 | 24 July 2019 |
| Rt Hon Theresa Anne Villiers | 24 July 2019 | 13 February 2020 |
| Charles George Eustice | 13 February 2020 | 6 September 2022 |